Agarose acrylamide two-dimentional electrophoresis. For total protein analysis of cells.
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چکیده
منابع مشابه
Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis.
Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus. However, the matrix of this low percentage gel is relatively weak, and one can separate the two forms of apoB in a slab gel apparatus only if on...
متن کاملProteome analysis of Cryptosporidium parvum and C. hominis using two-dimentional electrophoresis, image analysis and tandem mass spectrometry
Until recently, Cryptosporidium was thought to be a single species genus. Molecular studies now showthat there are at least 10 valid species of this parasite. Among them, two morphologically identical species, C.hominis and C. parvum are the most pathogenic identified to date and share 97% of identical genomes.Post-genomic analyses is therefore necessary to explore further the...
متن کاملA computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis.
We present a computer model to predict the patterns expected for the replication intermediates (RIs) of DNA fragments analyzed by neutral/neutral two-dimensional (2D) agarose gel electrophoresis. The model relies on the mode of replication (uni- or bi-directional), the electrophoretic mobility of linear DNA fragments and the retardation caused by the three-dimensional shape of non-linear molecu...
متن کاملAgarose gel electrophoresis.
MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...
متن کاملAgarose gel electrophoresis.
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 1988
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu1962.26.536